Stellaris

Configuration

Microscope:
Invers Nikon TiE

Focus hold system:
Ti-ND6-PFS-S Perfect Focus System

Transmittance illumination:
LED 100 coolwhite (can be triggered)

Stage:
Multiwell plate, 35 mm dish and slide format
200 µm z-focus Piezo nano drive

Environmental control:
Large Cage incubator CO2, humidity, heating

Objectives:
CFI P-Achromat 10X/ 0.25/
CFI Apo LWD 40x WI Lambda-S/ 1.15*
CFI P-Apo 40x Lambda/ 0.95*
CFI Apochromat TIRF 60x Öl/ 1.49/*
CFI P-Apo 100x Lambda Öl/ 1.45*

*Differential Interference Contrast (DIC) available

Laser light source:
Andor laser line combiner
Diode 405 nm
DPSS 488 nm
DPSS 561 nm
Diode 638 nm

Spinning disk confocal unit:
Yokogawa W1 spinning disk unit
50 µm disk pattern

Beamsplitter:
Quad band dichroic mirror 405/488/561/640
Pass 400-410, 483-493,557-563,633-643; Reflection 425-470,505-542,578-623, 660-750

Triple band dichroic mirror 445/515/594
Pass 440-450, 513-517,592-596; Reflection 463-497, 532-575, 613-750

Emission Filter:
Multi band
Semrock Brightline, Triple Band Fluorescence Filter, 440-40 / 521-21 / 607-34 / 700-45

Single band
525/50 nm
540/30 nm
600/50 nm
700/75 nm
Polarizer

Borrealis for homogenous illumination

Light stimulation and bleaching (FRAP, FLIP, photactivation)
Frappa with 70:30 quad dichroic 405/488/561/640

Camera:
ANDOR iXon DU-888U3-CS0-BV EMCCD camera
1024 x 1024 pixel
Pixel size 13 x 13 µm
QE >90%
Peltier cooling -85 °C

TIRF:
Nikon TIRF condenser with one motorized axis

Widefield Epifluorescence:

Light source:
LUMENCOR SOLA SE II

Filtercube: (HC BrightLine)
DAPI (ex 390/18; dm 416, em 460/60)
eGFP (ex 469/35, dm 497, em 525/39)
Cy3.5 (ex 565/24, dm 585, em 620/52

Software:
NIS elements
JOBS
Real Time Aquisition

Computer:
Xeon 6Core Prozessor
32 GB RAM
nVidia Quadro K2200 graphic card
512 GB SSD
4 TB SATA Festplatte